circrnas chip arraystar mouse circrnas chip  (Arraystar inc)

Journal: Computational and Structural Biotechnology Journal

Article Title: Identification of Differentially Expressed Profiles of Alzheimer's Disease Associated Circular RNAs in a Panax Notoginseng Saponins-Treated Alzheimer's Disease Mouse Model

doi: 10.1016/j.csbj.2018.10.010

Figure Lengend Snippet: Primers designed for qRT-PCR validation of candidate circRNAs.

Article Snippet: Three hippocampal tissues of model and PNS high-dosage group were used for microarray assay to measure differentially expressed circRNAs using the circRNAs chip (Arraystar mouse circRNAs chip, AraryStar) as indicated in our previous study [ ].The microarray hybridization including purifying RNA, transcribing into fluorescent cRNA was performed based on the manufacturer's standard protocols and then hybridizing onto mouse circRNA arrays.

Techniques: Biomarker Discovery

Journal: Computational and Structural Biotechnology Journal

Article Title: Identification of Differentially Expressed Profiles of Alzheimer's Disease Associated Circular RNAs in a Panax Notoginseng Saponins-Treated Alzheimer's Disease Mouse Model

doi: 10.1016/j.csbj.2018.10.010

Figure Lengend Snippet: The hierarchical cluster, scatter plot and volcano plot of differential expression of circRNAs in PNS-treated and –untreated SAMP8 mice. A: Hierarchical cluster of differentially expressed circRNAs. “Green” indicates low intensity, “black” indicates medium intensity and “red” indicates strong intensity. B: Scatter plot of circRNA signal values. The values of X and Y axes represents the normalized signal values of the samples (log2 scaled) and the averaged normalized signal values of samples (log2 scaled) respectively. The green lines are fold change lines. The CircRNAs above the top green line and below the bottom green line demonstrates >1.5-fold change of circRNAs between the two compared samples. C: Volcano plot of differential expression of circRNAs. The vertical lines correspond to 1.5-fold up and down, respectively. The horizontal line represents a P-value of 0.05, and the red point in the plot represents the differentially expressed circRNAs with statistical significance.

Article Snippet: Three hippocampal tissues of model and PNS high-dosage group were used for microarray assay to measure differentially expressed circRNAs using the circRNAs chip (Arraystar mouse circRNAs chip, AraryStar) as indicated in our previous study [ ].The microarray hybridization including purifying RNA, transcribing into fluorescent cRNA was performed based on the manufacturer's standard protocols and then hybridizing onto mouse circRNA arrays.

Techniques: Quantitative Proteomics

Journal: Computational and Structural Biotechnology Journal

Article Title: Identification of Differentially Expressed Profiles of Alzheimer's Disease Associated Circular RNAs in a Panax Notoginseng Saponins-Treated Alzheimer's Disease Mouse Model

doi: 10.1016/j.csbj.2018.10.010

Figure Lengend Snippet: The expression levels of candidate circRNAs for validation by qRT-PCR in 15 SAMP8 hippocampal tissues. Statistically differences were calculated by one-way ANOVA using SPSS 13.0 software. *P < 0.05, **P < 0.01 versus SAMP8 group.

Article Snippet: Three hippocampal tissues of model and PNS high-dosage group were used for microarray assay to measure differentially expressed circRNAs using the circRNAs chip (Arraystar mouse circRNAs chip, AraryStar) as indicated in our previous study [ ].The microarray hybridization including purifying RNA, transcribing into fluorescent cRNA was performed based on the manufacturer's standard protocols and then hybridizing onto mouse circRNA arrays.

Techniques: Expressing, Biomarker Discovery, Quantitative RT-PCR, Software